Epitope Mapping by NMR of a Novel Anti-Aβ Antibody (STAB-MAb).

ABSTRACT: Alzheimer´s Disease (AD) is one of the most common neurodegenerative disorders worldwide. Excess of β-amyloid (Aβ), a peptide with a high propensity to misfold and self-aggregate, is believed to be the major contributor to the observed neuronal degeneration and cognitive decline in AD. Here, we characterize the epitope of a novel anti-Aβ monoclonal antibody, the STAB-MAb, which has previously demonstrated picomolar affinities for both monomers (K_D = 80 pM) and fibrils (K_D = 130 pM) of Aβ(1-42) and has shown therapeutic efficacy in preclinical mouse models of AD. Our findings reveal a widespread epitope that embraces several key Aβ residues that have been previously described as important in the Aβ fibrillation process. Of note, STAB-MAb exhibits a stronger affinity fo

SUBMITTER: Posado-Fernandez A 

PROVIDER: S-EPMC6706428 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature