Depletion of the Transcriptional Coactivator Amplified in Breast Cancer 1 (AIB1) Uncovers Functionally Distinct Subpopulations in Triple-Negative Breast Cancer.

ABSTRACT: The transcriptional coactivator Amplified in Breast Cancer 1 (AIB1) plays a major role in the progression of hormone and HER2-dependent breast cancers but its role in triple negative breast cancer (TNBC) is undefined. Here, we report that established TNBC cell lines, as well as cells from a TNBC patient-derived xenograft (PDX) that survive chemotherapy treatment in vitro express lower levels of AIB1 protein. The surviving cell population has an impaired tube-formation phenotype when cultured onto basement membrane, a property shared with TNBC cells that survive shRNA-mediated depletion of AIB1 (AIB1^LOW cells). DNA analysis by exome sequencing revealed that AIB1^LOW cells represent a distinct subpopulation. Consistent with their in vitro phenotype AIB1^LOW cel

SUBMITTER: Saenz FR 

PROVIDER: S-EPMC6706655 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature