Enhancer Priming Enables Fast and Sustained Transcriptional Responses to Notch Signaling.

ABSTRACT: Information from developmental signaling pathways must be accurately decoded to generate transcriptional outcomes. In the case of Notch, the intracellular domain (NICD) transduces the signal directly to the nucleus. How enhancers decipher NICD in the real time of developmental decisions is not known. Using the MS2-MCP system to visualize nascent transcripts in single cells in Drosophila embryos, we reveal how two target enhancers read Notch activity to produce synchronized and sustained profiles of transcription. By manipulating the levels of NICD and altering specific motifs within the enhancers, we uncover two key principles. First, increased NICD levels alter transcription by increasing duration rather than frequency of transcriptional bursts. Second, priming of enhancers by tissue-spec

SUBMITTER: Falo-Sanjuan J 

PROVIDER: S-EPMC6706658 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature