Optimization and High Level Production of Recombinant Synthetic Streptokinase in E. coli Using Response Surface Methodology.

ABSTRACT: Streptokinase (SK) is an extracellular protein comprising 414 amino acids with considerable clinical importance as a commonly used thrombolytic agent. Due to its wide spread application and clinical importance designing more efficient SK production platforms worth investigation. In this regard, a synthetic SK gene was optimized and cloned in to pET21b plasmid for periplasmic expression. Response surface methodology was used to design a total of 20 experiments for optimization of IPTG concentration, post-induction period, and cell density of induction (OD₆₀₀). The optimum levels of the selected parameters were successfully determined to be 0.28 mM for IPTG concentration, 9.889 H for post induction period, and 3.40768 for cell density (OD₆₀₀). These settings result in 4

SUBMITTER: Aghaeepoor M 

PROVIDER: S-EPMC6706719 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature