Calbindin-D28K Limits Dopamine Release in Ventral but Not Dorsal Striatum by Regulating Ca²⁺ Availability and Dopamine Transporter Function.

ABSTRACT: The calcium-binding protein calbindin-D28K, or calb1, is expressed at higher levels by dopamine (DA) neurons originating in the ventral tegmental area (VTA) than in the adjacent substantia nigra pars compacta (SNc). Calb1 has received attention for a potential role in neuroprotection in Parkinson's disease. The underlying physiological roles for calb1 are incompletely understood. We used cre-loxP technology to knock down calb1 in mouse DA neurons to test whether calb1 governs axonal release of DA in the striatum, detected using fast-scan cyclic voltammetry ex vivo. In the ventral but not dorsal striatum, calb1 knockdown elevated DA release and modified the spatiotemporal coupling of Ca²⁺ entry to DA release. Furthermore, calb1 knockdown enhanced DA uptake but attenuated the impa

SUBMITTER: Brimblecombe KR 

PROVIDER: S-EPMC6706870 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature