Development of a real-time loop-mediated isothermal amplification assay for detection of porcine circovirus 3.

ABSTRACT: BACKGROUND:Porcine circovirus type 3 (PCV3) is an emerging circovirus species, that has been reported in major pig-raising countries including the United States, China, South Korea, Brazil, Spain, and Poland. RESULTS:A real-time loop-mediated isothermal amplification (LAMP) assay was developed for rapid detection of porcine circovirus 3 (PCV3). The method had a detection limit of 1?×?101 copies/?L with no cross-reactions with classical swine fever virus (CSFV) C strain, foot-and-mouth disease virus (FMDV), porcine circovirus 2 (PCV2) LG vaccine strain, porcine epidemic diarrhoea virus (PEDV), porcine respiratory and reproductive syndrome virus (PRRSV), or pseudorabies virus (PRV). The PCV3 positive detection rate of 203 clinical samples for the real-time LAMP assay was 89.66% (182/203). CONCLUSIONS:The real-time LAMP assay is highly sensitive, and specific for use in epidemiological investigations of PCV3.

SUBMITTER: Wang H 

PROVIDER: S-EPMC6706899 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature