Circulating cell-free DNA as a biomarker of tissue injury: Assessment in a cardiac xenotransplantation model.

ABSTRACT:

Background

Observational studies suggest that cell-free DNA (cfDNA) is a biomarker of tissue injury in a range of conditions including organ transplantation. However, the lack of model systems to study cfDNA and its relevance to tissue injury has limited the advancements in this field. We hypothesized that the predictable course of acute humoral xenograft rejection (AHXR) in organ transplants from genetically engineered donors provides an ideal system for assessing circulating cfDNA as a marker of tissue injury.

Methods

Genetically modified pig donor hearts were heterotopically transplanted into baboons (n = 7). Cell-free DNA was extracted from pre-transplant and post-transplant baboon plasma samples for shotgun sequencing. After alignment of sequence reads to pig and baboon

SUBMITTER: Agbor-Enoh S 

PROVIDER: S-EPMC6707066 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature