Automated real-time monitoring of human pluripotent stem cell aggregation in stirred tank reactors.

ABSTRACT: The culture of human induced pluripotent stem cells (hiPSCs) at large scale becomes feasible with the aid of scalable suspension setups in continuously stirred tank reactors (CSTRs). Innovative monitoring options and emerging automated process control strategies allow for the necessary highly defined culture conditions. Next to standard process characteristics such as oxygen consumption, pH, and metabolite turnover, a reproducible and steady formation of hiPSC aggregates is vital for process scalability. In this regard, we developed a hiPSC-specific suspension culture unit consisting of a fully monitored CSTR system integrated into a custom-designed and fully automated incubator. As a step towards cost-effective hiPSC suspension culture and to pave the way for flexibility at a large scale,

SUBMITTER: Schwedhelm I 

PROVIDER: S-EPMC6707254 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature