Nano-mechanical mapping of interdependent cell and ECM mechanics by AFM force spectroscopy.

ABSTRACT: Extracellular matrix (ECM), as a dynamic component of the tissue, influences cell behavior and plays an important role in cell mechanics and tissue homeostasis. Reciprocally, this three-dimensional scaffold is dynamically, structurally and mechanically modified by cells. In the field of biophysics, the independent role of cell and ECM mechanics has been largely investigated; however, there is a lack of experimental data reporting the interdependent interplay between cell and ECM mechanics, measured simultaneously. Here, using Atomic Force Microscopy (AFM) we have characterized five different decellularized matrices diverse in their topography, ECM composition and stiffness and cultured them with normal and pathological fibroblasts (scar and Dupuytren's). We investigated the change in topog

SUBMITTER: Viji Babu PK 

PROVIDER: S-EPMC6707266 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature