Improved methods for fluorescent labeling and detection of single extracellular vesicles using nanoparticle tracking analysis.

ABSTRACT: Growing interest in extracellular vesicles (EV) has necessitated development of protocols to improve EV characterization as a precursor for myriad downstream investigations. Identifying expression of EV surface epitopes can aid in determining EV enrichment and allow for comparisons of sample phenotypes. This study was designed to test a rigorous method of indirect fluorescent immunolabeling of single EV with subsequent evaluation using nanoparticle tracking analysis (NTA) to simultaneously determine EV concentration, particle size distribution, and surface immunophenotype. In this study, EV were isolated from canine and human cell cultures for immunolabeling and characterized using NTA, transmission electron microscopy, and Western blotting. Indirect fluorescent immunolabeling utilizing qu

SUBMITTER: Thane KE 

PROVIDER: S-EPMC6707283 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature