Proteomic analyses of Urine Exosomes reveal New Biomarkers of Diabetes in Pregnancy.

ABSTRACT:

Objective

To evaluate 24 hour urine exosome protein content changes among pregnant US subjects with diabetes and obesity during early pregnancy.

Methods

The exosome proteome content from 24 hour urine samples of pregnant subjects with gestational diabetes mellitus (GDM, N=8) and pre-gestational Type 2 diabetes (PGD, N = 10) were compared with control samples (CTRL, N = 10) obtained at week 20 of pregnancy. Differences in exosome protein load between groups was identified by liquid chromatography/mass spectrometry, analyzed by linear regression in negative binomial distribution, visualized in MetaboAnalyst (version 3.0), and validated by western immunoblotting.

Results

At the 20^th week of pregnancy, we identified 646, 734 and 856 proteins in exosomes from 24

SUBMITTER: Ramachandrarao SP 

PROVIDER: S-EPMC6707737 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature