C677T polymorphism increases the risk of early spontaneous abortion.

ABSTRACT:

Purpose

To investigate the relationship of the methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism in male population having spouses with early spontaneous abortion.

Methods

A total of 345 males whose spouses had at least one early spontaneous abortion were included in the study group, and 145 males who planned to have a second child were included in the control group. Semen was collected in a sterile cup by masturbation. After liquefaction, the sperm concentration and forward motility sperm rate (PR) were obtained. The genomic DNA was extracted from peripheral vein, followed by MTHFR C677T polymorphism detection through PCR-gold magnetic nanoparticle chromatography.

Results

The numbers of alleles and genotypes of MTHFR in the case group were 303 (C), 387 (

SUBMITTER: Liu Y 

PROVIDER: S-EPMC6707973 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature