Regulation of boar sperm functionality by the nitric oxide synthase/nitric oxide system.

ABSTRACT:

Purpose

Nitric oxide (NO) is a free radical synthesized mainly by nitric oxide synthases (NOSs). NO regulates many aspects in sperm physiology in different species. However, in vitro studies investigating NOS distribution, and how NO influences sperm capacitation and fertilization (IVF) in porcine, have been lacking. Therefore, our study aimed to clarify these aspects.

Methods

Two main experiments were conducted: (i) boar spermatozoa were capacitated in the presence/absence of S-nitrosoglutathione (GSNO), a NO donor, and two NOS inhibitors, N^G-nitro-L-arginine methyl ester hydrochloride (L-NAME) and aminoguanidine hemisulfate salt (AG), and (ii) IVF was performed in the presence or not of these supplements, but neither the oocytes nor the sperm were previously inc

SUBMITTER: Staicu FD 

PROVIDER: S-EPMC6707978 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature