Lipid profiling of suction blister fluid: comparison of lipids in interstitial fluid and plasma.

ABSTRACT:

Background

Recent technical advances in the extraction of dermal interstitial fluid (ISF) have stimulated interest in using this rather unexploited biofluid as an alternative to blood for detection and prediction of disease. However, knowledge about the presence of useful biomarkers for health monitoring in ISF is still limited. In this study, we characterized the lipidome of human suction blister fluid (SBF) as a surrogate for pure ISF and compared it to that of plasma.

Methods

Plasma and SBF samples were obtained from 18 healthy human volunteers after an overnight fast. Total lipids were extracted and analyzed by liquid chromatography-tandem mass spectrometry. One hundred ninety-three lipid species covering 10 complex lipid classes were detected and quantified in both plas

SUBMITTER: Nilsson AK 

PROVIDER: S-EPMC6708155 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature