A new primer construction technique that effectively increases amplification of rare mutant templates in samples.

ABSTRACT:

Background

In personalized medicine, companion diagnostic tests provide additional information to help select a treatment option likely to be optimal for a patient. Although such tests include several techniques for detecting low levels of mutant genes in wild-type backgrounds with fairly high sensitivity, most tests are not specific, and may exhibit high false positive rates. In this study, we describe a new primer structure, named 'stuntmer', to selectively suppress amplification of wild-type templates, and promote amplification of mutant templates.

Results

A single stuntmer for a defined region of DNA can detect several kinds of mutations, including point mutations, deletions, and insertions. Stuntmer PCRs are also highly sensitive, being able to amplify mutant sequences

SUBMITTER: Huang JK 

PROVIDER: S-EPMC6708177 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature