Characterization of an immortalized human small airway basal stem/progenitor cell line with airway region-specific differentiation capacity.

ABSTRACT:

Background

The pathology of chronic obstructive pulmonary disease (COPD), idiopathic pulmonary fibrosis (IPF) and most lung cancers involves the small airway epithelium (SAE), the single continuous layer of cells lining the airways ≥ 6th generations. The basal cells (BC) are the stem/progenitor cells of the SAE, responsible for the differentiation into intermediate cells and ciliated, club and mucous cells. To facilitate the study of the biology of the human SAE in health and disease, we immortalized and characterized a normal human SAE basal cell line.

Methods

Small airway basal cells were purified from brushed SAE of a healthy nonsmoker donor with a characteristic normal SAE transcriptome. The BC were immortalized by retrovirus-mediated telomerase reverse transcriptase (TE

SUBMITTER: Wang G 

PROVIDER: S-EPMC6708250 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature