Autophagy enhances mesenchymal stem cell-mediated CD4⁺ T cell migration and differentiation through CXCL8 and TGF-β1.

ABSTRACT:

Background

Mesenchymal stem cells (MSCs) have been recognized as a promising tool for the treatment of various inflammatory disorders and autoimmune diseases. Stress conditions affect immune-mediated treatment and activate autophagy in MSCs. However, whether autophagy affects the MSC-mediated recruitment and differentiation of CD4⁺ T cells remains elusive.

Methods

MSCs were pretreated with 3-methyladenine (3-MA) and rapamycin to regulate autophagy, and then co-cultured with CD4⁺ T cells. CD4⁺ T cell migration and differentiation were detected by flow cytometry. Further, gene expression levels of well-known chemokines were analyzed by quantitative real-time PCR. Enzyme-linked immunosorbent assays and western blot analysis were performed to det

SUBMITTER: Cen S 

PROVIDER: S-EPMC6708254 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature