Efficient Front-Rear Coupling in Neutrophil Chemotaxis by Dynamic Myosin II Localization.

ABSTRACT: Efficient chemotaxis requires rapid coordination between different parts of the cell in response to changing directional cues. Here, we investigate the mechanism of front-rear coordination in chemotactic neutrophils. We find that changes in the protrusion rate at the cell front are instantaneously coupled to changes in retraction at the cell rear, while myosin II accumulation at the rear exhibits a reproducible 9-15-s lag. In turning cells, myosin II exhibits dynamic side-to-side relocalization at the cell rear in response to turning of the leading edge and facilitates efficient turning by rapidly re-orienting the rear. These manifestations of front-rear coupling can be explained by a simple quantitative model incorporating reversible actin-myosin interactions with a rearward-flowing actin

SUBMITTER: Tsai TY 

PROVIDER: S-EPMC6708378 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature