Transcriptional profiling of intramembranous and endochondral ossification after fracture in mice.

ABSTRACT: Bone fracture repair represents an important clinical challenge with nearly 1 million non-union fractures occurring annually in the U.S. Gene expression differs between non-union and healthy repair, suggesting there is a pattern of gene expression that is indicative of optimal repair. Despite this, the gene expression profile of fracture repair remains incompletely understood. In this work, we used RNA-seq of two well-established murine fracture models to describe gene expression of intramembranous and endochondral bone formation. We used top differentially expressed genes, enriched gene ontology terms and pathways, callus cellular phenotyping, and histology to describe and contrast these bone formation processes across time. Intramembranous repair, as modeled by ulnar stress fracture, and

SUBMITTER: Coates BA 

PROVIDER: S-EPMC6708791 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature