Bacterial load and inflammatory response in sputum of alpha-1 antitrypsin deficiency patients with COPD.

ABSTRACT: Background:Airway inflammation may drive the progression of chronic obstructive pulmonary disease (COPD) associated with alpha-1 antitrypsin deficiency (AATD), but the relationship between airway microbiota and inflammation has not been investigated. Methods:We studied 21 non-treated AATD (AATD-noT) patients, 20 AATD-COPD patients under augmentation therapy (AATD-AT), 20 cigarette smoke-associated COPD patients, 20 control healthy smokers (CS) and 21 non-smokers (CON) with normal lung function. We quantified sputum inflammatory cells and inflammatory markers (IL-27, CCL3, CCL5, CXCL8, LTB4, MPO) by ELISA, total bacterial load (16S) and pathogenic bacteria by qRT-PCR. Results:AATD-AT patients were younger but had similar spirometric and DLCO values compared to cigarette smoke-associated COP

SUBMITTER: Balbi B 

PROVIDER: S-EPMC6709647 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature