Midgut barriers prevent the replication and dissemination of the yellow fever vaccine in Aedes aegypti.

ABSTRACT:

Background

To be transmitted to vertebrate hosts via the saliva of their vectors, arthropod-borne viruses have to cross several barriers in the mosquito body, including the midgut infection and escape barriers. Yellow fever virus (YFV) belongs to the genus Flavivirus, which includes human viruses transmitted by Aedes mosquitoes, such as dengue and Zika viruses. The live-attenuated YFV-17D vaccine has been used safely and efficiently on a large scale since the end of World War II. Early studies have shown, using viral titration from salivary glands of infected mosquitoes, that YFV-17D can infect Aedes aegypti midgut, but does not disseminate to other tissues.

Methodology/principal findings

Here, we re-visited this issue using a panel of techniques, such as RT-qPCR, Western bl

SUBMITTER: Danet L 

PROVIDER: S-EPMC6709925 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature