3D bioprinted mammary organoids and tumoroids in human mammary derived ECM hydrogels.

ABSTRACT: The extracellular matrix (ECM) of tissues is an important mediator of cell function. Moreover, understanding cellular dynamics within their specific tissue context is also important for developmental biology, cancer research, and regenerative medicine. However, robust in vitro models that incorporate tissue-specific microenvironments are lacking. Here we describe a novel mammary-specific culture protocol that combines a self-gelling hydrogel comprised solely of ECM from decellularized rat or human breast tissue with the use of our previously described 3D bioprinting platform. We initially demonstrate that undigested and decellularized mammary tissue can support mammary epithelial and tumor cell growth. We then describe a methodology for generating mammary ECM extracts that can spontaneousl

SUBMITTER: Mollica PA 

PROVIDER: S-EPMC6710129 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature