Construction of complete Tupaia belangeri transcriptome database by whole-genome and comprehensive RNA sequencing.

ABSTRACT: The northern tree shrew (Tupaia belangeri) possesses high potential as an animal model of human diseases and biology, given its genetic similarity to primates. Although genetic information on the tree shrew has already been published, some of the entire coding sequences (CDSs) of tree shrew genes remained incomplete, and the reliability of these CDSs remained difficult to determine. To improve the determination of tree shrew CDSs, we performed sequencing of the whole-genome, mRNA, and total RNA and integrated the resulting data. Additionally, we established criteria for the selection of reliable CDSs and annotated these sequences by comparison to the human transcriptome, resulting in the identification of complete CDSs for 12,612 tree shrew genes and yielding a more accurate tree shrew gen

SUBMITTER: Sanada T 

PROVIDER: S-EPMC6710255 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature