Fast LC-ESI-MS/MS analysis and influence of sampling conditions for gut metabolites in plasma and serum.

ABSTRACT: In the past few years, the gut microbiome has been shown to play an important role in various disorders including in particular cardiovascular diseases. Especially the metabolite trimethylamine-N-oxide (TMAO), which is produced by gut microbial metabolism, has repeatedly been associated with an increased risk for cardiovascular events. Here we report a fast liquid chromatography tandem mass spectrometry (LC-MS/MS) method that can analyze the five most important gut metabolites with regards to TMAO in three minutes. Fast liquid chromatography is unconventionally used in this method as an on-line cleanup step to remove the most important ion suppressors leaving the gut metabolites in a cleaned flow through fraction, also known as negative chromatography. We compared different blood matrix ty

SUBMITTER: van der Laan T 

PROVIDER: S-EPMC6710273 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature