Nonallelic homologous recombination events responsible for copy number variation within an RNA silencing locus.

ABSTRACT: The structure of chalcone synthase (CHS) gene repeats in different alleles of the I (inhibitor) locus in soybean spawns endogenous RNA interference (RNAi) that leads to phenotypic change in seed coat color of this major agronomic crop. Here, we examined CHS gene copy number by digital PCR and single nucleotide polymorphisms (SNPs) through whole genome resequencing of 15 cultivars that varied in alleles of the I locus (I, iⁱ , i^k , and i) that control the pattern distribution of pigments in the seed coats. Lines homozygous for the iⁱ allele had the highest copy number followed by the I and i^k cultivars which were more related to each other than to the lines with i

SUBMITTER: Cho YB 

PROVIDER: S-EPMC6710647 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature