Cell Type- and Exposure-Specific Modulation of CD63/CD81-Positive and Tissue Factor-Positive Extracellular Vesicle Release in response to Respiratory Toxicants.

ABSTRACT: Chronic exposure to respiratory stressors increases the risk for pulmonary and cardiovascular diseases. Previously, we have shown that cigarette smoke extract (CSE) triggers the release of CD63⁺CD81⁺ and tissue factor (TF)⁺ procoagulant extracellular vesicles (EVs) by bronchial epithelial cells via depletion of cell surface thiols. Here, we hypothesized that this represents a universal response for different pulmonary cell types and respiratory exposures. Using bead-based flow cytometry, we found that bronchial epithelial cells and pulmonary fibroblasts, but not pulmonary microvascular endothelial cells or macrophages, release CD63⁺CD81⁺ and TF⁺ EVs in response to CSE. Cell surface thiols decreased in all cell types upon CSE

SUBMITTER: Stassen FRM 

PROVIDER: S-EPMC6710792 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature