Sleeping beauty genetic screen identifies miR-23b::BTBD7 gene interaction as crucial for colorectal cancer metastasis.

ABSTRACT:

Background

Metastatic colorectal cancer (CRC) remains a deadly disease. Identifying locally advanced CRC patients with high risk of developing metastasis and improving outcome of metastatic CRC patients require discovering master regulators of metastasis. In this context, the non-coding part of the human genome is still largely unexplored.

Methods

To interrogate the non-coding part of the human genome and disclose regulators of CRC metastasis, we combined a transposon-based forward genetic screen with a novel in vitro assay, which forces cells to grow deprived of cell-substrate and cell-cell contacts (i.e. forced single cell suspension assay - fSCS).

Findings

We proved that fSCS selects CRC cells with mesenchymal and pro-metastatic traits. Moreover, we found that the

SUBMITTER: Grisard E 

PROVIDER: S-EPMC6710852 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature