Tobacco smoking induces changes in true DNA methylation, hydroxymethylation and gene expression in bronchoalveolar lavage cells.

ABSTRACT:

Background

While smoking is known to associate with development of multiple diseases, the underlying mechanisms are still poorly understood. Tobacco smoking can modify the chemical integrity of DNA leading to changes in transcriptional activity, partly through an altered epigenetic state. We aimed to investigate the impact of smoking on lung cells collected from bronchoalveolar lavage (BAL).

Methods

We profiled changes in DNA methylation (5mC) and its oxidised form hydroxymethylation (5hmC) using conventional bisulphite (BS) treatment and oxidative bisulphite treatment with Illumina Infinium MethylationEPIC BeadChip, and examined gene expression by RNA-seq in healthy smokers.

Findings

We identified 1667 total 5mC + 5hmC, 1756 5mC and 67 5hmC differentially methylated

SUBMITTER: Ringh MV 

PROVIDER: S-EPMC6710853 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature