Population-based inference of aminoglycoside resistance mechanisms in Escherichia coli.

ABSTRACT:

Background

Interpretative reading of antimicrobial susceptibility test (AST) results allows inferring biochemical resistance mechanisms from resistance phenotypes. For aminoglycosides, however, correlations between resistance pathways inferred on the basis of the European Committee on Antimicrobial Susceptibility Testing (EUCAST) clinical breakpoints and expert rules versus genotypes are generally poor. This study aimed at developing and validating a decision tree based on resistance phenotypes determined by disc diffusion and based on epidemiological cut-offs (ECOFFs) to infer the corresponding resistance mechanisms in Escherichia coli.

Methods

Phenotypic antibiotic susceptibility of thirty wild-type and 458 aminoglycoside-resistant E. coli clinical isolates was determined

SUBMITTER: Mancini S 

PROVIDER: S-EPMC6710905 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature