Genetic screen of the yeast environmental stress response dynamics uncovers distinct regulatory phases.

ABSTRACT: Cells respond to environmental fluctuations by regulating multiple transcriptional programs. This response can be studied by measuring the effect of environmental changes on the transcriptome or the proteome of the cell at the end of the response. However, the dynamics of the response reflect the working of the regulatory mechanisms in action. Here, we utilized a fluorescent stress reporter gene to track the dynamics of protein production in yeast responding to environmental stress. The response is modulated by changes in both the duration and rate of transcription. We probed the underlying molecular pathways controlling these two dimensions using a library of ~1,600 single- and double-mutant strains. Dissection of the effects of these mutants and the interactions between them identified d

SUBMITTER: Gutin J 

PROVIDER: S-EPMC6711295 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature