Allele specific DNA marker for fusarium resistance gene FocBo1 in Brassica oleracea.

ABSTRACT: The fusarium yellows resistance (YR) gene FocBo1 was previously identified and the DNA markers were developed to assist the breeding of YR cultivars in Brassica oleracea. However, the further analysis revealed discrepancies between the phenotypes and the genotypes predicted by those DNA markers in cabbage commercial cultivars. Since this discrepancy seemed to be due to unknown susceptible alleles of focbo1, we sequenced the gene in 19 accessions to determine the sequence variations between alleles and found that there were two resistant FocBo1 alleles and six susceptible alleles in the investigated population. The newly designed PCR markers detected three mutations in the susceptible alleles that generate premature termination codons. These were shown to accurat

SUBMITTER: Sato M 

PROVIDER: S-EPMC6711725 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature