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Brief Report: Cell Cycle Dynamics of Human Pluripotent Stem Cells Primed for Differentiation.

ABSTRACT: Understanding the molecular properties of the cell cycle of human pluripotent stem cells (hPSCs) is critical for effectively promoting differentiation. Here, we use the Fluorescence Ubiquitin Cell Cycle Indicator system adapted into hPSCs and perform RNA sequencing on cell cycle sorted hPSCs primed and unprimed for differentiation. Gene expression patterns of signaling factors and developmental regulators change in a cell cycle-specific manner in cells primed for differentiation without altering genes associated with pluripotency. Furthermore, we identify an important role for PI3K signaling in regulating the early transitory states of hPSCs toward differentiation. Stem Cells 2019;37:1151-1157.

SUBMITTER: Shcherbina A

PROVIDER: S-EPMC6711778 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Brief Report: Cell Cycle Dynamics of Human Pluripotent Stem Cells Primed for Differentiation.

Shcherbina Anna A Li Jingling J Narayanan Cyndhavi C Greenleaf William W Kundaje Anshul A Chetty Sundari S

Stem cells (Dayton, Ohio) 20190610 9

Understanding the molecular properties of the cell cycle of human pluripotent stem cells (hPSCs) is critical for effectively promoting differentiation. Here, we use the Fluorescence Ubiquitin Cell Cycle Indicator system adapted into hPSCs and perform RNA sequencing on cell cycle sorted hPSCs primed and unprimed for differentiation. Gene expression patterns of signaling factors and developmental regulators change in a cell cycle-specific manner in cells primed for differentiation without altering ...[more]

PMID: 31135093

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