Albumin evokes Ca²⁺-induced cell oxidative stress and apoptosis through TRPM2 channel in renal collecting duct cells reduced by curcumin.

ABSTRACT: In proteinuric nephropathies of chronic kidney disease, the epithelial cells of the nephron including the collecting duct are exposed to high concentrations of luminal albumin. Albumin is taken up from collecting duct cells by endocytosis causing excessive reactive oxygen species (ROS) production and a proinflammatory response. Curcumin used in the traditional medicine possesses anti-inflammatory and antioxidant effects. ROS and ADP-ribose (ADPR) activate the cation channel TRPM2. We hypothesize, that albumin-induced cell stress and proinflammatory response are mediated by Ca²⁺ and can be reduced by curcumin. The cortical collecting duct (CCD) cells mpkCCD_c14 exhibit spontaneous and inducible Ca²⁺ oscillations, which can be blocked by pre-treatment with cur

SUBMITTER: Nazıroglu M 

PROVIDER: S-EPMC6711968 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature