Precisely Control Mitochondria with Light to Manipulate Cell Fate Decision.

ABSTRACT: Mitochondrial dysfunction has been implicated in many pathological conditions and diseases. The normal functioning of mitochondria relies on maintaining the inner mitochondrial membrane potential (also known as ΔΨm) that is essential for ATP synthesis, Ca²⁺ homeostasis, redox balance, and regulation of other key signaling pathways such as mitophagy and apoptosis. However, the detailed mechanisms by which ΔΨm regulates cellular function remain incompletely understood, partially because of the difficulty of manipulating ΔΨm with spatiotemporal resolution, reversibility, or cell type specificity. To address this need, we have developed a next generation optogenetic-based technique for controllable mitochondrial depolarization with light. We demonstrate successful targeting of the h

SUBMITTER: Ernst P 

PROVIDER: S-EPMC6712847 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature