¹H-NMR metabolomics reveals the Glabrescione B exacerbation of glycolytic metabolism beside the cell growth inhibitory effect in glioma.

ABSTRACT:

Background

Glioma is the most common and primary brain tumors in adults. Despite the available multimodal therapies, glioma patients appear to have a poor prognosis. The Hedgehog (Hh) signaling is involved in tumorigenesis and emerged as a promising target for brain tumors. Glabrescione B (GlaB) has been recently identified as the first direct inhibitor of Gli1, the downstream effector of the pathway.

Methods

We established the overexpression of Gli1 in murine glioma cells (GL261) and GlaB effect on cell viability. We used ¹H-nuclear magnetic resonance (NMR) metabolomic approach to obtain informative metabolic snapshots of GL261 cells acquired at different time points during GlaB treatment. The activation of AMP activated protein Kinase (AMPK) induced by GlaB was

SUBMITTER: D'Alessandro G 

PROVIDER: S-EPMC6712882 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature