Next-generation sequencing predicts interaction network between miRNA and target genes in lipoteichoic acid-stimulated human neutrophils.

ABSTRACT: Toll‑like receptors (TLRs), which are a class of pattern‑recognition receptors, can sense specific molecules of pathogens and then activate immune cells, such as neutrophils. The regulation of TLR signaling in immune cells has been investigated by various studies. However, the interaction of TLR signaling‑activated microRNAs (miRNAs) and genes has not been well investigated in a specific type of immune cells. In the present study, neutrophils were isolated from peripheral blood of a healthy donor, and then treated for 16 h with Staphylococcus aureus lipoteichoic acid (LTA), which is an agonist of TLR2. The miRNA and mRNA expression profiles were analyzed via next‑generation sequencing and bioinformatics approaches. A total of 290 differentially expressed genes between LTA‑treated and vehic

SUBMITTER: Yen MC 

PROVIDER: S-EPMC6713436 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature