A Comprehensive Roadmap of Murine Spermatogenesis Defined by Single-Cell RNA-Seq.

ABSTRACT: Spermatogenesis requires intricate interactions between the germline and somatic cells. Within a given cross section of a seminiferous tubule, multiple germ and somatic cell types co-occur. This cellular heterogeneity has made it difficult to profile distinct cell types at different stages of development. To address this challenge, we collected single-cell RNA sequencing data from ∼35,000 cells from the adult mouse testis and identified all known germ and somatic cells, as well as two unexpected somatic cell types. Our analysis revealed a continuous developmental trajectory of germ cells from spermatogonia to spermatids and identified candidate transcriptional regulators at several transition points during differentiation. Focused analyses delineated four subtypes of spermatogonia and nine

SUBMITTER: Green CD 

PROVIDER: S-EPMC6713459 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature