Time-Resolved Small RNA Sequencing Unravels the Molecular Principles of MicroRNA Homeostasis.

ABSTRACT: Argonaute-bound microRNAs silence mRNA expression in a dynamic and regulated manner to control organismal development, physiology, and disease. We employed metabolic small RNA sequencing for a comprehensive view on intracellular microRNA kinetics in Drosophila. Based on absolute rate of biogenesis and decay, microRNAs rank among the fastest produced and longest-lived cellular transcripts, disposing up to 10⁵ copies per cell at steady-state. Mature microRNAs are produced within minutes, revealing tight intracellular coupling of biogenesis that is selectively disrupted by pre-miRNA-uridylation. Control over Argonaute protein homeostasis generates a kinetic bottleneck that cooperates with non-coding RNA surveillance to ensure faithful microRNA loading. Finally, regulated small RNA

SUBMITTER: Reichholf B 

PROVIDER: S-EPMC6713562 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature