LAG-3 expression on tumor-infiltrating T cells in soft tissue sarcoma correlates with poor survival.

ABSTRACT:

Objective

To elucidate the role and prognostic significance of lymphocyte activation-gene-3 (LAG-3) in soft tissue sarcoma (STS).

Methods

The expression of LAG-3 in patient and matched normal blood samples was analyzed by flow cytometry. The localization and prognostic values of LAG-3⁺ cells in 163 STS patients were analyzed by immunohistochemistry. In addition, the expression of tumor-infiltrating CD3⁺ T, CD4⁺ T, and CD8⁺ T cells and their role in the prognosis of STS were evaluated by immunohistochemistry. The effect of LAG-3 blockade was evaluated in an immunocompetent MCA205 fibrosarcoma mouse model.

Results

Peripheral CD8⁺ and CD4⁺ T cells from STS patients expressed higher levels of LAG-3 than tho

SUBMITTER: Que Y 

PROVIDER: S-EPMC6713642 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature