An anthocyanin marker for direct visualization of plant transformation and its use to study nitrogen-fixing nodule development.

ABSTRACT: The development and functioning of the nitrogen fixing symbiosis between legume plants and soil bacteria collectively called rhizobia requires continuous chemical dialogue between the partners using different molecules such as flavonoids, lipo-chitooligosaccharides, polysaccharides and peptides. Agrobacterium rhizogenes mediated hairy root transformation of legumes is widely used to study the function of plant genes involved in the process. The identification of transgenic plant tissues is based on antibiotics/herbicide selection and/or the detection of different reporter genes that usually require special equipment such as fluorescent microscopes or destructive techniques and chemicals to visualize enzymatic activity. Here, we developed and efficiently used in hairy root experiments binar

SUBMITTER: Zhang S 

PROVIDER: S-EPMC6713694 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature