A label-free optical whole-cell Escherichia coli biosensor for the detection of pyrethroid insecticide exposure.

ABSTRACT: There is a growing need for low-cost, portable technologies for the detection of threats to the environment and human health. Here we propose a label-free, optical whole-cell Escherichia coli biosensor for the detection of 3-phenoxybenzoic acid (3-PBA), a biomarker for monitoring human exposure to synthetic pyrethroid insecticides. The biosensor functions like a competitive ELISA but uses whole-cells surface displaying an anti-3-PBA VHH as the detection element. When the engineered cells are mixed with 3-PBA-protein conjugate crosslinking that can be visually detected occurs. Free 3-PBA in samples competes with these crosslinks, leading to a detectable change in the output. The assay performance was improved by coloring the cells via expression of the purple-blue amilCP chromoprotein and t

SUBMITTER: Riangrungroj P 

PROVIDER: S-EPMC6713742 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature