Analysis of redox landscapes and dynamics in living cells and in vivo using genetically encoded fluorescent sensors.

ABSTRACT: Cellular oxidation-reduction reactions are mainly regulated by pyridine nucleotides (NADPH/NADP⁺ and NADH/NAD⁺), thiols, and reactive oxygen species (ROS) and play central roles in cell metabolism, cellular signaling, and cell-fate decisions. A comprehensive evaluation or multiplex analysis of redox landscapes and dynamics in intact living cells is important for interrogating cell functions in both healthy and disease states; however, until recently, this goal has been limited by the lack of a complete set of redox sensors. We recently reported the development of a series of highly responsive, genetically encoded fluorescent sensors for NADPH that substantially strengthen the existing toolset of genetically encoded sensors for thiols, H₂O₂, and N

SUBMITTER: Zou Y 

PROVIDER: S-EPMC6714056 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature