Characterization of a whole blood assay for quantifying myeloid-derived suppressor cells.

ABSTRACT:

Background

Myeloid-derived suppressor cells (MDSC) have been found to play an important role in limiting immune responses in cancer. Higher circulating MDSC levels have been associated with greater tumor burden, poorer response to immunotherapy, and poorer survival. Optimal measurement of MDSC levels could provide clinicians with a useful prognostic and/or management tool.

Methods

A whole blood (WB) nine color, 11 parameter flow cytometric assay was designed, utilizing fluorescently-labeled antibodies against CD45, CD3, CD19, CD20, CD56, CD16, HLA-DR, CD33, CD11b, CD14 and CD15, and BD Trucount beads for quantitation. Total MDSC were defined as CD45 + CD3^-CD19^-CD20^-CD56^-CD16^-HLA-DR^-CD33 + CD11b + cells, while

SUBMITTER: Apodaca MC 

PROVIDER: S-EPMC6714080 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature