The RNA-Binding Protein KSRP Modulates Cytokine Expression of CD4⁺ T Cells.

ABSTRACT: The KH-type splicing regulatory protein (KSRP) is a RNA-binding protein, which regulates the stability of many mRNAs encoding immune-relevant proteins. As KSRP regulates innate immune responses, for instance by the modulation of type I interferon mRNA stability, we were interested whether knockdown of the protein (KSRP^-/-) interferes with T cell activation and polarization. Polyclonally stimulated KSRP^-/- CD4⁺ T cells proliferated at a higher extent and higher frequency and expressed the activation marker CD25 more than wild-type T cells. In supernatants of stimulated KSRP^-/- CD4⁺ T cells, levels of IL-5, IL-9, IL-10, and IL-13 were observed to be increased compared to those of the control group. KSRP^-/- CD8⁺ T ce

SUBMITTER: Kafer R 

PROVIDER: S-EPMC6714327 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature