Determination of growth-coupling strategies and their underlying principles.

ABSTRACT:

Background

Metabolic coupling of product synthesis and microbial growth is a prominent approach for maximizing production performance. Growth-coupling (GC) also helps stabilizing target production and allows the selection of superior production strains by adaptive laboratory evolution. To support the implementation of growth-coupling strain designs, we seek to identify biologically relevant, metabolic principles that enforce strong growth-coupling on the basis of reaction knockouts.

Results

We adapted an established bilevel programming framework to maximize the minimally guaranteed production rate at a fixed, medium growth rate. Using this revised formulation, we identified various GC intervention strategies for metabolites of the central carbon metabolism, which were examin

SUBMITTER: Alter TB 

PROVIDER: S-EPMC6714386 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature