Stable distinct core eukaryotic viromes in different mosquito species from Guadeloupe, using single mosquito viral metagenomics.

ABSTRACT:

Background

Mosquitoes are the most important invertebrate viral vectors in humans and harbor a high diversity of understudied viruses, which has been shown in many mosquito virome studies in recent years. These studies generally performed metagenomics sequencing on pools of mosquitoes, without assessment of the viral diversity in individual mosquitoes. To address this issue, we applied our optimized viral metagenomics protocol (NetoVIR) to compare the virome of single and pooled Aedes aegypti and Culex quinquefasciatus mosquitoes collected from different locations in Guadeloupe, in 2016 and 2017.

Results

The total read number and viral reads proportion of samples containing a single mosquito have no significant difference compared with those of pools containing five mosquito

SUBMITTER: Shi C 

PROVIDER: S-EPMC6714450 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature