Enhanced xeno-free differentiation of hiPSC-derived astroglia applied in a blood-brain barrier model.

ABSTRACT:

Background

Human induced pluripotent stem cells (hiPSC) hold great promise for use in cell therapy applications and for improved in vitro models of human disease. So far, most hiPSC differentiation protocols to astroglia use undefined, animal-containing culture matrices. Laminins, which play an essential role in the regulation of cell behavior, offer a source of defined, animal-free culture matrix.

Methods

In order to understand how laminins affect astroglia differentiation, recombinant human laminin-521 (LN521), was compared to a murine Engelbreth-Holm-Swarm sarcoma derived laminin (L2020). Astroglia expression of protein and mRNA together with glutamate uptake and protein secretion function, were evaluated. Finally, these astroglia were evaluated in a coculture model of th

SUBMITTER: Delsing L 

PROVIDER: S-EPMC6714544 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature