Model System for the Formation of Tick-Borne Encephalitis Virus Replication Compartments without Viral RNA Replication.

ABSTRACT: Flavivirus is a positive-sense, single-stranded RNA viral genus, with members causing severe diseases in humans such as tick-borne encephalitis, yellow fever, and dengue fever. Flaviviruses are known to cause remodeling of intracellular membranes into small cavities, where replication of the viral RNA takes place. Nonstructural (NS) proteins are not part of the virus coat and are thought to participate in the formation of these viral replication compartments (RCs). Here, we used tick-borne encephalitis virus (TBEV) as a model for the flaviviruses and developed a stable human cell line in which the expression of NS proteins can be induced without viral RNA replication. The model system described provides a novel and benign tool for studies of the viral components under controlled exp

SUBMITTER: Yau WL 

PROVIDER: S-EPMC6714791 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature