Metabolic switches from quiescence to growth in synchronized Saccharomyces cerevisiae.

ABSTRACT:

Introduction

The switch from quiescence (G0) into G1 and cell cycle progression critically depends on specific nutrients and metabolic capabilities. Conversely, metabolic networks are regulated by enzyme-metabolite interaction and transcriptional regulation that lead to flux modifications to support cell growth. How cells process and integrate environmental information into coordinated responses is challenging to analyse and not yet described quantitatively.

Objectives

To quantitatively monitor the central carbon metabolism during G0 exit and the first 2 h after reentering the cell cycle from synchronized Saccharomyces cerevisiae.

Methods

Dynamic tailored ¹³C metabolic flux analysis was used to observe the intracellular metabolite flux changes, and the meta

SUBMITTER: Zhang J 

PROVIDER: S-EPMC6715666 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature