EGR1 recruits TET1 to shape the brain methylome during development and upon neuronal activity.

ABSTRACT: Life experience can leave lasting marks, such as epigenetic changes, in the brain. How life experience is translated into storable epigenetic information remains largely unknown. With unbiased data-driven approaches, we predicted that Egr1, a transcription factor important for memory formation, plays an essential role in brain epigenetic programming. We performed EGR1 ChIP-seq and validated thousands of EGR1 binding sites with methylation patterns established during postnatal brain development. More specifically, these EGR1 binding sites become hypomethylated in mature neurons but remain heavily methylated in glia. We further demonstrated that EGR1 recruits a DNA demethylase TET1 to remove the methylation marks and activate downstream genes. The frontal cortices from the knockout mice lack

SUBMITTER: Sun Z 

PROVIDER: S-EPMC6715719 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature